5XOW

Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 6'A7' on the target strand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	298
Detector technology	CCD
Collection date	2015-05-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 3 2
Unit cell lengths	200.824, 200.824, 200.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.707 - 2.902
R-factor	0.2164
Rwork	0.214
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kpy
RMSD bond length	0.008
RMSD bond angle	1.476
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge		0.528
Number of reflections	30962	1504
<I/σ(I)>	15.8	2.1
Completeness [%]	99.3	99
Redundancy	8.8	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		308	2.0 M Na-formate and 0.1 M Tris-HCl pH 7.2-7.5