5XOJ
Crystal structure of Xpo1p-PKI-Nup42p-Gsp1p-GTP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-04 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.640, 107.560, 149.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.582 - 2.200 |
R-factor | 0.1924 |
Rwork | 0.191 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wyg |
RMSD bond length | 0.002 |
RMSD bond angle | 0.656 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.1.27) |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.582 | 31.580 | 2.240 |
High resolution limit [Å] | 2.200 | 11.430 | 2.200 |
Rmerge | 0.119 | 0.032 | 0.798 |
Rpim | 0.057 | 0.016 | 0.403 |
Number of reflections | 81567 | ||
<I/σ(I)> | 9.4 | 25.4 | 2 |
Completeness [%] | 97.8 | 93.7 | 96.4 |
Redundancy | 5.2 | 4.7 | 4.7 |
CC(1/2) | 0.996 | 0.998 | 0.560 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | PEG20000, Tris-HCl |