5XN6
Heterodimer crystal structure of geranylgeranyl diphosphate synthases 1 with GGPPS Recruiting Protein(OsGRP) from Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2013-09-10 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 |
| Unit cell lengths | 103.062, 102.981, 103.486 |
| Unit cell angles | 109.39, 109.59, 109.13 |
Refinement procedure
| Resolution | 37.755 - 3.598 |
| R-factor | 0.2525 |
| Rwork | 0.252 |
| R-free | 0.26330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e8h |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.542 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.800 |
| High resolution limit [Å] | 3.590 |
| Number of reflections | 36920 |
| <I/σ(I)> | 8.26 |
| Completeness [%] | 96.9 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 16% PEG 10000, 0.1 M Bis-Tris, pH 6.5 |
