5XN5
Homo-dimer crystal structure of geranylgeranyl diphosphate synthases 1 from Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2013-09-10 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.022, 74.118, 71.288 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 38.056 - 2.002 |
| R-factor | 0.1884 |
| Rwork | 0.187 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e8h |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.374 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.100 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 39062 |
| <I/σ(I)> | 19 |
| Completeness [%] | 99.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 2.0 M sodium malonate, 20% PEG 3350, pH 7.0 |
