5XMY

Crystal structure of TAF3 PHD finger bound to H3K4me3Q5ser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2015-03-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	26.982, 48.096, 52.700
Unit cell angles	90.00, 98.16, 90.00

Refinement procedure

Resolution	25.274 - 1.699
R-factor	0.1835
Rwork	0.182
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c13
RMSD bond length	0.006
RMSD bond angle	0.915
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2689)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.699	4.610	1.700
Rmerge	0.069	0.046	0.856
Number of reflections	14658
<I/σ(I)>	14.2
Completeness [%]	99.2	96.9	99.4
Redundancy	5.1	4.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.03 M magnesium chloride, 0.03 M calcium chloride, 0.1 M MES, 0.1 M imidazole, PH6.5, 15% PEGMME 550, 15% PEG 20K