5XJF
Crystal structure of fucosylated IgG Fc Y296W mutant complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.300, 77.300, 349.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.22277 |
| Rwork | 0.220 |
| R-free | 0.26991 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ay4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.609 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.077 | 0.902 |
| Number of reflections | 37895 | 5925 |
| <I/σ(I)> | 23.7 | 2.6 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 13.5 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 12% PEG 20000, 0.1 M MES (pH 6.5), 4% Zwittergent 3-14 |
