5XJF

Crystal structure of fucosylated IgG Fc Y296W mutant complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Immunoglobulin gamma-1 heavy chain	polymer	223	25120.5	2	UniProt (P0DOX5) Pfam (PF07654) In PDB	Homo sapiens (Human)	Immunoglobulin gamma-1 heavy chain NIE
2	C	Low affinity immunoglobulin gamma Fc region receptor III-A	polymer	179	20641.0	1	UniProt (P08637) Pfam (PF13895) In PDB	Homo sapiens (Human)	CD16a antigen,Fc-gamma RIII-alpha,FcRIIIa,FcR-10,IgG Fc receptor III-2
3	D, E	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1625.5	2	In PDB GlyTouCan (G27919IH)
4	F	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		951.9	1	In PDB GlyTouCan (G10133VD)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
7		water	water		18.0	109	Chemie (HOH)

Sequence modifications

A, B: 225 - 447 (UniProt: P0DOX5)

PDB	External Database	Details
Trp 296	Tyr 298	engineered mutation

C: 3 - 175 (UniProt: P08637)

PDB	External Database	Details
Gln 38	Asn 56	engineered mutation
Gln 74	Asn 92	engineered mutation
Val 158	Phe 176	engineered mutation
Gln 169	Asn 187	engineered mutation
His 176	-	expression tag
His 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	70881.9
Branched	Number of molecules	3
	Total formula weight	4202.9
Non-Polymers*	Number of molecules	2
	Total formula weight	256.7
All*	Total formula weight	75341.5

*Water molecules are not included.