5XJE
Crystal structure of fucosylated IgG1 Fc complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.550, 77.550, 351.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.23514 |
| Rwork | 0.233 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ay4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.573 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.104 | 0.611 |
| Number of reflections | 43217 | 6663 |
| <I/σ(I)> | 14.2 | 1.6 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 11.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 12% PEG 20000, 0.1 M MES (pH 6.5), 4% Zwittergent 3-12 |
