5XGP

structure of Sizzled from Xenopus laevis at 2.08 angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	70.131, 70.131, 132.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.855 - 2.077
R-factor	0.2143
Rwork	0.213
R-free	0.23000
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.457
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.080	2.080
Rmerge	0.071	0.956
Rpim	0.032	0.423
Number of reflections	20594	1014
<I/σ(I)>	30.1	3.1
Completeness [%]	99.3	99.8
Redundancy	6	6.1
CC(1/2)		0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	10% PEG8K, 0.1 M imidazole (pH 8.0) and 0.2 M lithium sulfate.