5XF5
Nucleosome core particle with an adduct of a binuclear RAPTA (Ru-arene-phosphaadamantane) compound having a 1,2-diphenylethylenediamine linker (R,S-configuration)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.210, 109.670, 182.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.650 - 2.820 |
| R-factor | 0.22847 |
| Rwork | 0.228 |
| R-free | 0.26351 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mnn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.197 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.650 | 2.970 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.502 | |
| Number of reflections | 52474 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 35-55mM MnCl2, 25-49mM KCl, 20mM K-Cacodylate pH 6.0 |
