5XDR
Crystal structure of human DEAH-box RNA helicase DHX15 in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-04 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.919, 89.377, 211.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.540 - 2.000 |
| R-factor | 0.22523 |
| Rwork | 0.223 |
| R-free | 0.26911 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.656 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 105.900 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 31906 |
| <I/σ(I)> | 18.4 |
| Completeness [%] | 100.0 |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG4000, lithium sulfate, sodium acetate |
