5X6Z
Crystal structure of Rice Dwarf Virus P5 in complex with GTP and GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-26 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 |
Unit cell lengths | 77.394, 83.447, 141.979 |
Unit cell angles | 103.10, 101.98, 95.69 |
Refinement procedure
Resolution | 37.659 - 2.100 |
R-factor | 0.1869 |
Rwork | 0.184 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5x6x |
RMSD bond length | 0.008 |
RMSD bond angle | 0.882 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.069 | 0.437 |
Number of reflections | 193037 | 9561 |
<I/σ(I)> | 19.2 | 3.4 |
Completeness [%] | 98.6 | 97.7 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 3350, 0.1M MES, pH 6.0 |