5X5V

Crystal structure of pseudorabies virus glycoprotein D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	79.398, 60.975, 60.681
Unit cell angles	90.00, 111.22, 90.00

Refinement procedure

Resolution	23.531 - 1.500
R-factor	0.1703
Rwork	0.169
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c36
RMSD bond length	0.006
RMSD bond angle	1.090
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rpim		0.129
Number of reflections	42212
<I/σ(I)>	25.5
Completeness [%]	97.6
Redundancy	6.1
CC(1/2)		0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1 M ammonium acetate 0.1 M bis-tris pH 5.5 17 % PEG 10,000