5X4Z
RNA Polymerase II from Komagataella Pastoris (Type-1 crystal)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-05 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 155.048, 160.351, 254.354 |
Unit cell angles | 90.00, 105.43, 90.00 |
Refinement procedure
Resolution | 49.820 - 7.800 |
R-factor | 0.3533 |
Rwork | 0.353 |
R-free | 0.35710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.819 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2614) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 8.080 |
High resolution limit [Å] | 7.800 | 16.620 | 7.800 |
Rmerge | 0.378 | 0.198 | |
Rmeas | 0.407 | 0.234 | |
Rpim | 0.270 | 0.123 | 0.838 |
Total number of observations | 51715 | ||
Number of reflections | 13973 | ||
<I/σ(I)> | 2.2 | ||
Completeness [%] | 99.9 | 99.8 | 100 |
Redundancy | 3.7 | 3.5 | 3.8 |
CC(1/2) | 0.961 | 0.423 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium citrate, 8% PGA-LM, 10% glycerol |