5X3K

Kfla1895 D451A mutant in complex with isomaltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	180.584, 180.584, 392.291
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.420 - 2.500
R-factor	0.1823
Rwork	0.182
R-free	0.21600
RMSD bond length	0.006
RMSD bond angle	0.957
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.420
High resolution limit [Å]	2.500
Number of reflections	85003
<I/σ(I)>	22.3
Completeness [%]	99.9
Redundancy	20.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.8 M lithium sulfate, 0.1 M imidazole