5X0T
Crystal structure of CD147 C2 domain in complex with Fab of its monoclonal antibody 6H8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.986, 83.486, 130.193 |
| Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
| Resolution | 37.161 - 2.500 |
| R-factor | 0.2485 |
| Rwork | 0.246 |
| R-free | 0.29990 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.919 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.340 | 2.620 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.289 | |
| Number of reflections | 33828 | |
| <I/σ(I)> | 20.1 | 7.1 |
| Completeness [%] | 97.0 | 84.9 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 0.2M lithium sulfate monohydrate, 20% (w/v) PEG3350 |
