5WZY
Crystal structure of the P2X4 receptor from zebrafish in the presence of CTP at 2.8 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 129.242, 129.242, 251.825 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.101 - 2.799 |
| R-factor | 0.2165 |
| Rwork | 0.213 |
| R-free | 0.24860 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.568 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.799 | 7.590 | 2.800 |
| Rmerge | 0.119 | 0.053 | 0.938 |
| Number of reflections | 20237 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 99.8 | 98.1 | 98.8 |
| Redundancy | 7.8 | 8.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 3 mM CTP, 20-26% PEG 2000, 300 mM Mg(NO3)2, 100 mM Tris |
