5WXH

Crystal structure of TAF3 PHD finger bound to H3K4me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	27.244, 35.138, 38.562
Unit cell angles	82.96, 75.62, 73.49

Refinement procedure

Resolution	37.296 - 1.297
R-factor	0.1541
Rwork	0.153
R-free	0.17510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.320
High resolution limit [Å]	1.297	3.530	1.300
Rmerge	0.071	0.060	0.521
Number of reflections	31171
<I/σ(I)>	15.7
Completeness [%]	95.5	98.9	93.4
Redundancy	5.1	6.3	4.3
CC(1/2)		0.995	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.03 M magnesium chloride, 0.03 M calcium chloride, 0.1 M MES, 0.1 M imidazole, PH6.5, 15% PEGMME 550, 15% PEG 20K