5WX9
Crystal Structure of AtERF96 with GCC-box
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.976, 81.181, 38.999 |
| Unit cell angles | 90.00, 120.04, 90.00 |
Refinement procedure
| Resolution | 21.120 - 1.760 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.773 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.051 | 0.341 |
| Number of reflections | 20152 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 97.3 | 98.9 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 297 | 0.02M Magnesium chloride hexahydrate &, 0.05M MOPS pH 7.0 &, 55% Tacsimate pH 7.0 |
