5WVO
Crystal structure of DNMT1 RFTS domain in complex with K18/K23 mono-ubiquitylated histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-11-08 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.362, 198.779, 76.904 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.181 - 1.997 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq 3epz |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.934 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.181 | 2.030 |
| High resolution limit [Å] | 1.997 | 2.000 |
| Rmerge | 0.706 | |
| Number of reflections | 35892 | |
| <I/σ(I)> | 6.7 | 3.6 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 6.4 | 6.1 |
| CC(1/2) | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 100mM Bis-Tris (pH 6.0), 200mM lithium sulfate monohydrate, 20% PEG 10000 |
