5WUP

Crystal structure of a insect group III chitinase (CAD1) from Ostrinia furnacalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	71.835, 71.835, 193.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.963 - 2.300
R-factor	0.1897
Rwork	0.188
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ybt
RMSD bond length	0.008
RMSD bond angle	1.109
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.963	2.420
High resolution limit [Å]	2.300	2.300
Number of reflections	23352
<I/σ(I)>	17.18
Completeness [%]	99.4	100
Redundancy	10.6	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	200 mM ammonium sulfate, 100 mM bis-Tris, 20% PEG3350