5WTP
Crystal structure of the C-terminal domain of outer membrane protein A (OmpA) from Capnocytophaga gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 130.366, 130.366, 56.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.191 - 2.150 |
| R-factor | 0.1844 |
| Rwork | 0.182 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fby |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.708 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.191 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 26492 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 97.6 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 100 mM Tris-HCl, pH 8.0, 3 M (NH4)2SO4 |
