5WSK

Structure of Ribulose-1,5-bisphosphate carboxylase/oxygenase from wheat

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2013-11-26
Detector	RAYONIX MX225-HS
Wavelength(s)	0.979
Spacegroup name	P 4
Unit cell lengths	110.720, 110.720, 200.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.415 - 1.781
R-factor	0.1562
Rwork	0.154
R-free	0.18740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uzh
RMSD bond length	0.012
RMSD bond angle	1.333
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.415
High resolution limit [Å]	1.780
Number of reflections	226185
<I/σ(I)>	1.34
Completeness [%]	98.4
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	0.1M Tris-HCl, pH 8.5, 12.5% PEG 3350