5WSC
Crystal of pyruvate kinase (PYK) from Mycobacterium tuberculosis in complex with Oxalate, soaked with allosteric activators AMP and Glucose 6-Phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-28 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 31 |
Unit cell lengths | 124.370, 124.370, 144.192 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.850 - 2.400 |
R-factor | 0.18162 |
Rwork | 0.179 |
R-free | 0.22696 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wrp |
RMSD bond length | 0.014 |
RMSD bond angle | 1.528 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.850 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 97539 | |
<I/σ(I)> | 8.3 | 1.6 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 5.5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 12% PEG 8000, 20% glycerol, 50 mM triethanolamine-HCl (TEA) buffer pH 7.2, 100 mM KCl, 50 mM MgCl2, 5 mM oxalate, 5 mM ATP, 5 mM AMP, 5 mM D-glucose 6-phosphate |