5WRJ
Crystal structure of human tyrosylprotein sulfotransferase-1 complexed with PAP and gastrin peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.668, 93.263, 93.012 |
| Unit cell angles | 90.60, 93.39, 103.39 |
Refinement procedure
| Resolution | 48.990 - 2.310 |
| R-factor | 0.2424 |
| Rwork | 0.241 |
| R-free | 0.26943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wri |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.330 |
| Number of reflections | 71093 |
| <I/σ(I)> | 8.33 |
| Completeness [%] | 97.5 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M potassium sodium tartrate trihydrate, 19.5% w/v polyethylene glycol 3350 |
