5WQE
Crystal structure of Alicyclobacillus acidoterrestris C2c1 in complex with single-guide RNA at 3.1 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9875 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.554, 129.836, 84.001 |
| Unit cell angles | 90.00, 110.00, 90.00 |
Refinement procedure
| Resolution | 48.642 - 3.126 |
| R-factor | 0.2391 |
| Rwork | 0.238 |
| R-free | 0.26810 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.893 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.135 | 0.658 |
| Number of reflections | 27598 | |
| <I/σ(I)> | 14.3 | 2.2 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 9.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M Tris-HCl, pH 8.0, 0.2 M LiCl and 16% PEG 8000 |
