5WPM
KRas G12V, bound to GppNHp and miniprotein 225-11(A30R)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.934, 37.810, 70.179 |
Unit cell angles | 90.00, 103.38, 90.00 |
Refinement procedure
Resolution | 68.280 - 1.720 |
R-factor | 0.2372 |
Rwork | 0.235 |
R-free | 0.28510 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.161 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 68.300 |
High resolution limit [Å] | 1.720 |
Number of reflections | 27343 |
<I/σ(I)> | 9.2 |
Completeness [%] | 98.9 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M calcium chloride and 20% PEG3350 |