5WNO
Crystal structure of C. elegans LET-23 kinase domain complexed with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.097, 77.978, 106.207 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.936 - 2.386 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rix |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.455 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.000 | 2.460 |
| High resolution limit [Å] | 2.386 | 2.386 |
| Rmerge | 0.100 | 0.890 |
| Number of reflections | 16320 | |
| <I/σ(I)> | 10.8 | 1.1 |
| Completeness [%] | 98.8 | 98.5 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG5000 MME, 0.20 M sodium chloride, 0.10 M HEPES, pH 7.5 |
