5WNJ

Crystal structure of murine receptor-interacting protein kinase 4 (Ripk4) D143N in complex with lestaurtinib

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	I 21 21 21
Unit cell lengths	70.825, 110.247, 145.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.550
R-factor	0.22105
Rwork	0.220
R-free	0.25226
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.232
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.010
High resolution limit [Å]	2.550
Rmerge	0.083
Number of reflections	18791
<I/σ(I)>	19.1
Completeness [%]	99.6
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		292	0.1M HEPES pH 7.5, 0.2M magnesium chloride, 10% 2-propanol, 15% ethylene glycol, 10% PEG4000