5WJ4

Crystal structure of redox-sensitive green fluorescent protein Clover mutant roClover1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	134.570, 134.570, 69.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.185 - 1.631
R-factor	0.1651
Rwork	0.164
R-free	0.19310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qyq
RMSD bond length	0.016
RMSD bond angle	1.365
Data reduction software	HKL-2000
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.190	39.190	1.660
High resolution limit [Å]	1.630	8.930	1.630
Rmerge	0.113	0.052	2.115
Rmeas	0.117	0.054	2.195
Rpim	0.031	0.015	0.584
Number of reflections	79222
<I/σ(I)>	11.2
Completeness [%]	100.0	98.8	99.9
Redundancy	14.6	12.1	14
CC(1/2)	0.999	0.997	0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	100mM Tris, pH 8.5, 50mM MgCl2, 25% PEG3350