5WIE

Crystal structure of a Kv1.2-2.1 chimera K+ channel V406W mutant in an inactivated state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 4 21 2
Unit cell lengths	143.124, 143.124, 284.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.300
Rwork	0.236
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r9r
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.330
Rpim	0.110	0.413
Number of reflections	45103
<I/σ(I)>	7.9	1.7
Completeness [%]	99.5	97.2
Redundancy	11.7	9
CC(1/2)	0.985	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	50 mM Tris-Cl pH 8.3, 29-31% PEG400