5WIE
Crystal structure of a Kv1.2-2.1 chimera K+ channel V406W mutant in an inactivated state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 143.124, 143.124, 284.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.300 |
| Rwork | 0.236 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r9r |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.330 | |
| Rpim | 0.110 | 0.413 |
| Number of reflections | 45103 | |
| <I/σ(I)> | 7.9 | 1.7 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 11.7 | 9 |
| CC(1/2) | 0.985 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 50 mM Tris-Cl pH 8.3, 29-31% PEG400 |
