5WI9
Crystal structure of KL with an agonist Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.018, 68.465, 147.811 |
| Unit cell angles | 90.00, 111.86, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.2183 |
| Rwork | 0.216 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.253 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.158 | 0.770 |
| Rmeas | 0.185 | 1.073 |
| Rpim | 0.095 | 0.547 |
| Number of reflections | 60307 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.987 | 0.617 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG 3350, 0.2M sodium sulfate |
