5WI4

CRYSTAL STRUCTURE OF DYNLT1/TCTEX-1 IN COMPLEX WITH ARHGEF2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-15
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9791
Spacegroup name	P 32 2 1
Unit cell lengths	91.967, 91.967, 83.079
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.830 - 2.000
R-factor	0.176
Rwork	0.174
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ygt
RMSD bond length	0.007
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	Coot ((0.7))
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.078	0.348
Number of reflections	27423
<I/σ(I)>	31.492	6.578
Completeness [%]	100.0	100
Redundancy	10.8	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	2.33M AMMONIUM SULPHATE, 0.25MM SODIUM MALONATE, 7.2MM CALCIUM CHLORIDE