5WHA
KRas G12V, bound to GDP and miniprotein 225-11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.003, 40.328, 230.939 |
Unit cell angles | 90.00, 90.99, 90.00 |
Refinement procedure
Resolution | 115.450 - 2.040 |
R-factor | 0.24 |
Rwork | 0.238 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gft |
RMSD bond length | 0.002 |
RMSD bond angle | 0.461 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 115.450 | |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.125 | |
Number of reflections | 55395 | |
<I/σ(I)> | 7.7 | |
Completeness [%] | 97.0 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.25 M CALCIUM CHLORIDE, 24% PEG 3350 |