5WH1
Apo form of the C-terminal region of human Transcription Factor IIB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-07-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.165, 126.461, 158.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.730 - 3.390 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.730 | 3.460 |
| High resolution limit [Å] | 3.390 | 3.394 |
| Rmerge | 0.239 | 0.000 |
| Rpim | 0.960 | |
| Number of reflections | 30686 | 30686 |
| <I/σ(I)> | 9.15 | 1.2 |
| Completeness [%] | 99.6 | 97.4 |
| Redundancy | 7.2 | 7.2 |
| CC(1/2) | 0.865 | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 100 mM sodium acetate trihydrate pH 4.5, 2 M ammonium sulfate |
