5WFN

Revised model of leiomodin 2-mediated actin regulation (alternate refinement of PDB 4RWT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	93
Detector technology	CCD
Collection date	2014-07-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1
Unit cell lengths	65.350, 65.650, 81.920
Unit cell angles	101.29, 90.94, 107.97

Refinement procedure

Resolution	44.245 - 3.000
R-factor	0.2101
Rwork	0.208
R-free	0.24630
RMSD bond length	0.009
RMSD bond angle	0.888
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.070
High resolution limit [Å]	2.980	2.980
Number of reflections	25186
Completeness [%]	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	20% PEG3350, 0.2 M magnesium acetate