5WEA
Human Argonaute2 Helix-7 Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.261, 107.880, 68.723 |
| Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
| Resolution | 65.555 - 3.120 |
| R-factor | 0.2537 |
| Rwork | 0.252 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ola |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.570 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.110 | 0.400 |
| Number of reflections | 14855 | |
| <I/σ(I)> | 9 | 2.4 |
| Completeness [%] | 94.5 | 96.8 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG 3350, 0.1 M phenol, 12% isopropanol, 0.1 M tris, pH 9.0 |
