5WEA
Human Argonaute2 Helix-7 Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.261, 107.880, 68.723 |
Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
Resolution | 65.555 - 3.120 |
R-factor | 0.2537 |
Rwork | 0.252 |
R-free | 0.28220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ola |
RMSD bond length | 0.002 |
RMSD bond angle | 0.570 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.000 | 3.200 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.110 | 0.400 |
Number of reflections | 14855 | |
<I/σ(I)> | 9 | 2.4 |
Completeness [%] | 94.5 | 96.8 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG 3350, 0.1 M phenol, 12% isopropanol, 0.1 M tris, pH 9.0 |