5WDQ

H-Ras mutant L120A bound to GMP-PNP at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	87.960, 87.960, 133.105
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.368 - 1.250
R-factor	0.1407
Rwork	0.140
R-free	0.15000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3k8y
RMSD bond length	0.009
RMSD bond angle	1.089
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.370	1.270
High resolution limit [Å]	1.250	1.250
Rmerge	0.045	0.594
Rpim	0.010	0.308
Number of reflections	54274	2444
<I/σ(I)>	36.9	2.4
Completeness [%]	99.0	92
Redundancy	18.7	4.6
CC(1/2)	1.000	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	17% PEG8000, 200 mM calcium acetate, 100 mM MES/NaOH, pH 6.0