5WDM
An ultra-stable single-chain insulin analog resists thermal inactivation and exhibits biological signaling duration equivalent to the native protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.12709 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.382, 85.771, 45.696 |
| Unit cell angles | 90.00, 110.89, 90.00 |
Refinement procedure
| Resolution | 38.220 - 2.803 |
| R-factor | 0.2572 |
| Rwork | 0.251 |
| R-free | 0.31420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1guj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS (Oct 15) |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.220 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.056 | 0.309 |
| Number of reflections | 7718 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M magnesium chloride, 15% PEG 8000, and 0.1 M Tris HCl |
