5WDK
A processive dipeptidyl aminopeptidase secreted from an established commensal bacterium P. distasonis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.912, 136.969, 208.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.100 - 2.360 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pw3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.924 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.100 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.230 | 0.957 |
| Rpim | 0.060 | 0.400 |
| Number of reflections | 120727 | 5972 |
| <I/σ(I)> | 13.9 | 2.11 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.5 | 6.8 |
| CC(1/2) | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.25 M K/Na tartrate, 17.5% PEG3350, 1 mM ZnCl2 |
