5WBH

Structure of the FRB domain of mTOR bound to a substrate recruitment peptide of S6K1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 2 21 21
Unit cell lengths	60.613, 80.944, 134.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 1.750
R-factor	0.1937
Rwork	0.193
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fap
RMSD bond length	0.007
RMSD bond angle	1.066
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge	0.068	0.040	0.603
Rmeas	0.076	0.045	0.680
Rpim	0.032	0.019	0.304
Total number of observations	328879
Number of reflections	66487		6153
<I/σ(I)>	11.6
Completeness [%]	98.2	97.3	92.4
Redundancy	4.9	5	4
CC(1/2)		0.998	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	tacsimate