5WB8

Crystal structure of the epidermal growth factor receptor extracellular region in complex with epigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-09
Detector	MARRESEARCH
Wavelength(s)	1.03
Spacegroup name	P 43 2 2
Unit cell lengths	104.050, 104.050, 285.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.889 - 3.000
R-factor	0.2683
Rwork	0.265
R-free	0.31160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EGFR domains I and III from PDB entry 3NJP
RMSD bond length	0.002
RMSD bond angle	0.654
Data reduction software	HKL-3000
Data scaling software	PHENIX
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.040	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.280	0.655
Rpim	0.141	0.290
Number of reflections	31787	3148
<I/σ(I)>	6.7	2.3
Completeness [%]	97.2	98.75
Redundancy	5.3	5.8
CC(1/2)		0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	0.1 magnesium formate, 15% PEG3350