5W9S

Zinc finger of human CXXC5 in complex with CpG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-08-22
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 32
Unit cell lengths	40.110, 40.110, 81.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.740 - 2.100
R-factor	0.2202
Rwork	0.218
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hp3
RMSD bond length	0.015
RMSD bond angle	1.744
Data reduction software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.740	34.740	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.040	0.021	1.013
Rmeas	0.042	0.022	1.061
Rpim	0.012	0.007	0.315
Total number of observations	96584	1196	7833
Number of reflections	8535
<I/σ(I)>	34.9	112	2.6
Completeness [%]	100.0	99.7	100
Redundancy	11.3	10.3	11.1
CC(1/2)	1.000	1.000	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	30% PEG-5000-MME, 0.2M ammonium sulfate, 0.1M MES