5W82

Crystal structure of Orsay virus delta protein N-terminal fragment (aa 1-101)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	77
Detector technology	CCD
Collection date	2016-06-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	55.746, 58.220, 163.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.030 - 1.800
R-factor	0.188
Rwork	0.186
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jie
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2152)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.107	0.833
Rmeas	0.122	0.042
Rpim	0.058	0.019
Total number of observations	196759
Number of reflections	49567
<I/σ(I)>	4.5
Completeness [%]	98.6	95.7
Redundancy	4	3.1
CC(1/2)		0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M Ammonium sulfate, 0.1M Sodium acetate pH 4.6, 25% (w/w) PEG 4000