5JIE
Crystal structure of the Orsay virus delta protein N-terminal fragment (aa 1~66)
Summary for 5JIE
| Entry DOI | 10.2210/pdb5jie/pdb |
| Descriptor | Protein delta (2 entities in total) |
| Functional Keywords | delta protein, orsay virus, c. elegans, helical, viral protein |
| Biological source | Orsay virus |
| Total number of polymer chains | 5 |
| Total formula weight | 36691.54 |
| Authors | |
| Primary citation | Fan, Y.,Guo, Y.R.,Yuan, W.,Zhou, Y.,Holt, M.V.,Wang, T.,Demeler, B.,Young, N.L.,Zhong, W.,Tao, Y.J. Structure of a pentameric virion-associated fiber with a potential role in Orsay virus entry to host cells. PLoS Pathog., 13:e1006231-e1006231, 2017 Cited by PubMed Abstract: Despite the wide use of Caenorhabditis elegans as a model organism, the first virus naturally infecting this organism was not discovered until six years ago. The Orsay virus and its related nematode viruses have a positive-sense RNA genome, encoding three proteins: CP, RdRP, and a novel δ protein that shares no homology with any other proteins. δ can be expressed either as a free δ or a CP-δ fusion protein by ribosomal frameshift, but the structure and function of both δ and CP-δ remain unknown. Using a combination of electron microscopy, X-ray crystallography, computational and biophysical analyses, here we show that the Orsay δ protein forms a ~420-Å long, pentameric fiber with an N-terminal α-helical bundle, a β-stranded filament in the middle, and a C-terminal head domain. The pentameric nature of the δ fiber has been independently confirmed by both mass spectrometry and analytical ultracentrifugation. Recombinant Orsay capsid containing CP-δ shows protruding long fibers with globular heads at the distal end. Mutant viruses with disrupted CP-δ fibers were generated by organism-based reverse genetics. These viruses were found to be either non-viable or with poor infectivity according to phenotypic and qRT-PCR analyses. Furthermore, addition of purified δ proteins to worm culture greatly reduced Orsay infectivity in a sequence-specific manner. Based on the structure resemblance between the Orsay CP-δ fiber and the fibers from reovirus and adenovirus, we propose that CP-δ functions as a cell attachment protein to mediate Orsay entry into worm intestine cells. PubMed: 28241071DOI: 10.1371/journal.ppat.1006231 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1374 Å) |
Structure validation
Download full validation report
