5W7F
Murine acyloxyacyl hydrolase (AOAH), S262A mutant, with lipid A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-04 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.797, 88.354, 93.450 |
| Unit cell angles | 90.00, 103.01, 90.00 |
Refinement procedure
| Resolution | 46.216 - 2.800 |
| R-factor | 0.2186 |
| Rwork | 0.216 |
| R-free | 0.26510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.536 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 31850 |
| <I/σ(I)> | 14.1 |
| Completeness [%] | 100.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 mM Triton X-100, 1 mM E. coli lipid A; 100 mM sodium MES pH 6, 15% PEG 20000 |
