5W7C

Human acyloxyacyl hydrolase (AOAH), proteolytically processed, S263A mutant, with LPS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-23
Detector	RAYONIX MX-300
Wavelength(s)	0.97243
Spacegroup name	P 21 21 21
Unit cell lengths	88.170, 104.080, 145.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.816 - 2.230
R-factor	0.2132
Rwork	0.211
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.621
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.230
Number of reflections	61346
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	post-chymotrypsin; 1mM Triton X-100, 0.333 mM E. coli LPS Ra; 200 mM ammonium chloride, 100 mM sodium acetate pH 5.3, 20 % PEG 6000