5W7B

Rabbit acyloxyacyl hydrolase (AOAH), proteolytically processed, S262A mutant, with LPS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-17
Detector	RAYONIX MX-300
Wavelength(s)	0.9801
Spacegroup name	P 21 21 2
Unit cell lengths	109.522, 138.806, 89.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.765 - 1.900
R-factor	0.1855
Rwork	0.185
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.982
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	106913
<I/σ(I)>	19.5
Completeness [%]	100.0
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	post-trypsin; 1 mM Triton X-100, 0.333 mM E. coli LPS Ra; 100 mM sodium MES pH 6; 34 % PEG 200; 5 % PEG 3000