5W7A

Rabbit acyloxyacyl hydrolase (AOAH), proteolytically processed, S262A mutant, with LPS (low quality saposin domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-23
Detector	RAYONIX MX-300
Wavelength(s)	0.97243
Spacegroup name	C 2 2 21
Unit cell lengths	89.578, 123.199, 125.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.686 - 2.300
R-factor	0.2412
Rwork	0.240
R-free	0.26150
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.746
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	31484
<I/σ(I)>	45
Completeness [%]	100.0
Redundancy	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	post-trypsin; 1 mM Triton X-100, 0.333 mM E. coli LPS Ra; 1 M sodium citrate pH 7