5W74

Crystal Structure of the Group II Chaperonin from Methanococcus Maripaludis D386ADeltaLid Mutant in the Open, ADP-Bound State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 21 2 21
Unit cell lengths	152.720, 221.930, 262.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.589 - 3.650
R-factor	0.2653
Rwork	0.265
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.531
Data reduction software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.589	3.710
High resolution limit [Å]	3.650	3.650
Number of reflections	96789	4255
<I/σ(I)>	11.3	1.5
Completeness [%]	97.4	88.7
Redundancy	15.5	11.5
CC(1/2)	0.997	0.329

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M MES pH 6.5, 5mM spermi- dine, 30% 2-Methyl-2,4-pentanediol